Journal: Journal of Antimicrobial Chemotherapy

Article Title: Reduced ceftazidime and ertapenem susceptibility due to production of OXA-2 in Klebsiella pneumoniae ST258

doi: 10.1093/jac/dkz183

Figure Lengend Snippet: Etest MICs for the OXA-2-producing K. pneumoniae isolate 1761 and corresponding E. coli TOP10 and K. pneumoniae ATCC 13883 transformants

Article Snippet: 11 This bla OXA-2 -carrying plasmid was only present in isolate 1761 and not in the other two isolates subjected to WGS, including isolate 1701, which shared only 17 non-recombinant SNPs with isolate 1761, suggesting that the p1761_02 plasmid was acquired by the ST258 K. pneumoniae strain during the course of the patient’s illness. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Isolate bla OXA-2 MIC (mg/L) ETP MEM IPM FEP CAZ K. pneumoniae 1761 + 0.38 0.094 0.38 0.25 4 E. coli TOP10 [pBC-SK(−)] − 0.008 0.023 0.38 0.064 0.25 E. coli TOP10 (p1761_02) + 0.023 0.032 0.38 0.064 4 E. coli TOP10 (pOXA-2) + 0.016 0.032 0.38 0.064 4 K. pneumoniae ATCC 13883 [pBC-SK(−)] − 0.008 0.023 0.75 0.64 0.38 K. pneumoniae ATCC 13883 (pOXA-2) + 0.125 0.094 0.94 6 Open in a separate window ETP, ertapenem; MEM, meropenem; IPM, imipenem; FEP, cefepime; CAZ, ceftazidime.

Techniques:

Journal: Cell

Article Title: Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2

doi: 10.1016/j.cell.2020.03.051

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD34+ human HSPCs were enriched with CD34 MicroBead Kit, human (130–046-702, Miltenyi Biotec), and cultured in StemMACS HSC Expansion Media XF, human (130–100-473, Miltenyi Biotec) supplemented with StemMACS HSC Expansion Cocktail, human (130–100-843, Miltenyi Biotec).

Techniques: Control, Western Blot, Immunohistochemistry, Flow Cytometry, Virus, Recombinant, Immunoprecipitation, IP Phosphatase Assay, Modification, Polymerization Assay, Fluorescence, Transgenic Assay, CRISPR, shRNA, Cloning, Plasmid Preparation, Selection, Software, Mutagenesis, Protease Inhibitor, Expressing, Cell Culture, Staining, Reporter Assay, Microscopy, Multiplex sample analysis, Protein Quantitation, Mass Spectrometry, Sample Prep, Phospho-proteomics